单细胞核测序技术是在单细胞水平上发展起来的技术,该技术可将冻存样本直接制核捕获,且依旧保存单细胞测序的高分辨率,能够准确区分细胞异质性,揭示单个细胞的基因结构和基因表达状态。因其对样本包容性高,核测序的出现解决了某些珍贵冻存样本,异形细胞样本无法进行单细胞实验的问题,在心肺疾病,神经发育等领域助力临床治疗和诊断,加速精准医疗时代。
1. 突破性改进制核手段,跳过样本消化步骤,冻存组织直接制核
2. 消除因消化偏好性造成的细胞解离偏差,且保证制核质量远高于上机捕获要求
3. 烈冰全程严格地质量把控,提供从实验设计到分析产出的一站式服务流程
样本类型:
1.冻存组织、培养的细胞系等,一般取黄豆粒大小;
2.-80℃冰箱保存,送样时干冰运输
Zhang, et al. Science Bulletin, 2020 Dec.
注:图为猕猴脑组织细胞亚群鉴定t-SNE图展示
Zhang, et al. Science Bulletin, 2020 Dec.
注:Marker基因的Violin图(左)和bubble plot图(右)
Zhang, et al. Science Bulletin, 2020 Dec.
注:该图为不同细胞亚群间差异基因聚类分析图(Heatmap)
Zhang, et al. Science Bulletin, 2020 Dec.
Zhang, et al. Science Bulletin, 2020 Dec.
Zhang C, et al. J Immunother Cancer, 2021;9:e002312.
注:细胞间状态转换的pesudotime轨迹图(左)和Heatmap图(右)
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